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rnjA [2018-01-11 09:06:43]
RNase J1, important for the degradation of RNA fragments containing transcription terminators
Molecular weight
61.34 kDa
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,523,118 → 1,524,785
Phenotypes of a mutant
growth is strongly impaired (doubling time in complex medium 145 minutes vs. 43 minutes for the wild type) PubMedthe mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMedit is not possible to construct a rnjA rny double mutant PubMed The protein
Catalyzed reaction/ biological activity
Protein family
RNase J subfamily (according to Swiss-Prot)Paralogous protein(s)
Effectors of protein activity
Structure
Localization
cytoplasm, colocalizes with the ribosomes PubMed Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMedrequired for thrS RNA processing, involved in maturation of the 5’-end of the 16S rRNA Expression and Regulation
Biological materials
Mutant
Expression vector
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's labfor chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's labpGP1441: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab LacZ fusion
GFP fusion
GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMedGP1722 (in pBP43), expression of rnjA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed FLAG-tag construct
Labs working on this gene/protein
References
Reviews
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Original publications
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