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rnjA [2018-01-11 09:06:43]
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rnjA [2018-01-11 09:06:43]

RNase J1, important for the degradation of RNA fragments containing transcription terminators
Locus
BSU14530
Isoelectric point
5.90
Molecular weight
61.34 kDa
Protein length
555 aa Sequence Blast
Gene length
1665 bp Sequence Blast
Function
RNA processing
Product
RNase J1
Essential
no
Synonyms
ykqC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
  • Groups of genes → Essential genes

    • Gene

    Coordinates
    1,523,118 → 1,524,785

    Phenotypes of a mutant

  • growth is strongly impaired (doubling time in complex medium 145 minutes vs. 43 minutes for the wild type) PubMed
  • the mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMed
  • it is not possible to construct a rnjA rny double mutant PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • endonuclease and 5'-3' exonuclease
  • degrades SR5 and gapA RNAs PubMed
  • degrades yflS mRNA PubMed

    • Protein family

  • RNase J subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • RnjB

    • Effectors of protein activity

  • initeraction with GapA-Sr1 stimulates activity PubMed

    • Structure

  • 3ZQ4 PubMed

    • Localization

  • cytoplasm, colocalizes with the ribosomes PubMed

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • required for thrS RNA processing, involved in maturation of the 5’-end of the 16S rRNA

    • Expression and Regulation

    Operons

    Genes
    rpoY-rnjA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
  • SSB342 (rnjA under pspac), cat, available in Harald Putzer lab
  • GP2506 (ΔrnjA::spc), available in Jörg Stülke's lab

    • Expression vector

  • for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
  • for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab
  • pGP1441: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

    • LacZ fusion

  • pGP418 (in pAC7), available in Jörg Stülke's lab

    • GFP fusion

  • GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
  • GP1722 (in pBP43), expression of rnjA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed

    • FLAG-tag construct

  • GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
  • GP1075 (aphA3), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Harald Putzer, IBPC Paris, France Homepage
  • David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
  • Ciaran Condon, IBPC, Paris, France Homepage

    • References

    Reviews

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    Original publications

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